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anti rabbit phospho p44 42 map kinase antibody  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti rabbit phospho p44 42 map kinase antibody
    Anti Rabbit Phospho P44 42 Map Kinase Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 16851 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+p44+42+map+kinase/Phospho-p44%2F42+MAPK+(Erk1%2F2)+(Thr202%2FTyr204)+Antibody/pm19191692__ja808387g_si_001-267-9-15
    Average 99 stars, based on 16851 article reviews
    anti rabbit phospho p44 42 map kinase antibody - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Cell-Signaling:

    Article Title: ATIP1 Is a Suppressor of Cardiac Hypertrophy and Modulates AT2-Dependent Signaling in Cardiac Myocytes
    Article Snippet: Subsequently, the rabbit serum was affinity purified with the antigen peptide coupled to an AF–Amino Toyopearl 650 M matrix (Tosoh Bioscience, Stuttgart, Germany). .. The blots were probed using anti-ATIP1, rabbit anti-p44/42 MAP kinase (9102, Cell Signaling), rabbit anti-phospho-p44/42 MAP kinase (9101, Cell Signaling), rabbit anti-GAPDH (14C10), rabbit anti-Cav1.2 (ACC-003, Alomone Labs), monoclonal mouse anti-PLB A1 (A010-14, Badrilla), monoclonal mouse anti-cardiac actin (A9357, Sigma, St. Louis, MO, USA), polyclonal rabbit anti-SERCA2a (A010-20, Badrilla, Leeds, UK), and monoclonal mouse anti-CaMKII (MA1-047, Pierce, Thermo Fisher Scientific, Hampton, NH, USA) antibodies, followed by an anti-rabbit or anti-mouse horseradish-peroxidase-conjugated secondary antibody (TrueBlot , eBioscience, San Diego, CA, USA), all diluted in 5% non-fat milk/PBS supplemented with 0.05% Tween 20. .. For signal detection, the ECL Plus reagent (Amersham, Freiburg, Germany) was used.

    Article Title: ATIP1 Is a Suppressor of Cardiac Hypertrophy and Modulates AT2-Dependent Signaling in Cardiac Myocytes.
    Article Snippet: Subsequently, the rabbit serum was affinity purified with the antigen peptide coupled to an AF–Amino Toyopearl 650 M matrix (Tosoh Bioscience, Stuttgart, Germany). .. The blots were probed using anti-ATIP1, rabbit anti-p44/42 MAP kinase (9102, Cell Signaling), rabbit anti-phospho-p44/42 MAP kinase (9101, Cell Signaling), rabbit anti-GAPDH (14C10), rabbit anti-Cav1.2 (ACC-003, Alomone Labs), monoclonal mouse anti-PLB A1 (A010-14, Badrilla), monoclonal mouse anti-cardiac actin (A9357, Sigma, St. Louis, MO, USA), polyclonal rabbit anti-SERCA2a (A010-20, Badrilla, Leeds, UK), and monoclonal mouse anti-CaMKII (MA1-047, Pierce, Thermo Fisher Scientific, Hampton, USA) antibodies, followed by an anti-rabbit or anti-mouse horseradish-peroxidase-conjugated secondary antibody (TrueBlot, eBioscience, San Diego, CA, USA), all diluted in 5% non-fat milk/PBS supplemented with 0.05% Tween 20. .. For signal detection, the ECL Plus reagent (Amersham, Freiburg, Germany) was used.

    Blocking Assay:

    Article Title: Eucommia ulmoides Oliv. Bark Extracts Alleviate MCAO/Reperfusion-Induced Neurological Dysfunction by Suppressing Microglial Inflammation in the Gray Matter.
    Article Snippet: An equivalent quantity of proteins (20 μg) was isolated on 10% sodium dodecyl sulfatepolyacrylamide gel electrophoresis and subsequently transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore, Darmstadt, Germany). .. Then, 5% skimmed milk was made to block the membranes at room temperature for 2 h. After that, the membranes were incubated with the following primary antibodies at 4◦C overnight: rabbit anti-TLR4 (#ab13556, 1:1000), rabbit anti-NF-κB (#ab16502, 1:1000) (purchased from Abcam (Cambridge, MA, USA)); rabbit anti-p38 (#9212S, 1:1000), rabbit anti-phosphorylated p38 (p-p38, #4511S, 1:1000), rabbit anti-p44/42 MAP kinase (ERK1/2, #9102S, 1:1000) and rabbit anti-phosphorylated p44/42 MAP kinase (p-ERK1/2, #4370S, 1:1000) (purchased from Cell Signaling Technology (Danvers, MA, USA)). .. After being rinsed with TBST, the membranes were incubated at room temperature for 2 h with horseradish peroxidase-conjugated goat anti-mouse IgG antibody or goat anti-rabbit IgG antibody (1:5000, Cell Signaling Technology (Danvers, MA, USA)) and imprinted with an enhanced chemiluminescence (ECL) detection kit (GE Healthcare, Buckinghamshire, UK).

    Article Title: Eucommia ulmoides Oliv. Bark Extracts Alleviate MCAO/Reperfusion-Induced Neurological Dysfunction by Suppressing Microglial Inflammation in the Gray Matter
    Article Snippet: An equivalent quantity of proteins (20 μg) was isolated on 10% sodium dodecyl sulfatepolyacrylamide gel electrophoresis and subsequently transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore, Darmstadt, Germany). .. Then, 5% skimmed milk was made to block the membranes at room temperature for 2 h. After that, the membranes were incubated with the following primary antibodies at 4°C overnight: rabbit anti-TLR4 (#ab13556, 1:1000), rabbit anti-NF-κB (#ab16502, 1:1000) (purchased from Abcam (Cambridge, MA, USA)); rabbit anti-p38 (#9212S, 1:1000), rabbit anti-phosphorylated p38 (p-p38, #4511S, 1:1000), rabbit anti-p44/42 MAP kinase (ERK1/2, #9102S, 1:1000) and rabbit anti-phosphorylated p44/42 MAP kinase (p-ERK1/2, #4370S, 1:1000) (purchased from Cell Signaling Technology (Danvers, MA, USA)). .. After being rinsed with TBST, the membranes were incubated at room temperature for 2 h with horseradish peroxidase-conjugated goat anti-mouse IgG antibody or goat anti-rabbit IgG antibody (1:5000, Cell Signaling Technology (Danvers, MA, USA)) and imprinted with an enhanced chemiluminescence (ECL) detection kit (GE Healthcare, Buckinghamshire, UK).

    Incubation:

    Article Title: Eucommia ulmoides Oliv. Bark Extracts Alleviate MCAO/Reperfusion-Induced Neurological Dysfunction by Suppressing Microglial Inflammation in the Gray Matter.
    Article Snippet: An equivalent quantity of proteins (20 μg) was isolated on 10% sodium dodecyl sulfatepolyacrylamide gel electrophoresis and subsequently transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore, Darmstadt, Germany). .. Then, 5% skimmed milk was made to block the membranes at room temperature for 2 h. After that, the membranes were incubated with the following primary antibodies at 4◦C overnight: rabbit anti-TLR4 (#ab13556, 1:1000), rabbit anti-NF-κB (#ab16502, 1:1000) (purchased from Abcam (Cambridge, MA, USA)); rabbit anti-p38 (#9212S, 1:1000), rabbit anti-phosphorylated p38 (p-p38, #4511S, 1:1000), rabbit anti-p44/42 MAP kinase (ERK1/2, #9102S, 1:1000) and rabbit anti-phosphorylated p44/42 MAP kinase (p-ERK1/2, #4370S, 1:1000) (purchased from Cell Signaling Technology (Danvers, MA, USA)). .. After being rinsed with TBST, the membranes were incubated at room temperature for 2 h with horseradish peroxidase-conjugated goat anti-mouse IgG antibody or goat anti-rabbit IgG antibody (1:5000, Cell Signaling Technology (Danvers, MA, USA)) and imprinted with an enhanced chemiluminescence (ECL) detection kit (GE Healthcare, Buckinghamshire, UK).

    Article Title: Eucommia ulmoides Oliv. Bark Extracts Alleviate MCAO/Reperfusion-Induced Neurological Dysfunction by Suppressing Microglial Inflammation in the Gray Matter
    Article Snippet: An equivalent quantity of proteins (20 μg) was isolated on 10% sodium dodecyl sulfatepolyacrylamide gel electrophoresis and subsequently transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore, Darmstadt, Germany). .. Then, 5% skimmed milk was made to block the membranes at room temperature for 2 h. After that, the membranes were incubated with the following primary antibodies at 4°C overnight: rabbit anti-TLR4 (#ab13556, 1:1000), rabbit anti-NF-κB (#ab16502, 1:1000) (purchased from Abcam (Cambridge, MA, USA)); rabbit anti-p38 (#9212S, 1:1000), rabbit anti-phosphorylated p38 (p-p38, #4511S, 1:1000), rabbit anti-p44/42 MAP kinase (ERK1/2, #9102S, 1:1000) and rabbit anti-phosphorylated p44/42 MAP kinase (p-ERK1/2, #4370S, 1:1000) (purchased from Cell Signaling Technology (Danvers, MA, USA)). .. After being rinsed with TBST, the membranes were incubated at room temperature for 2 h with horseradish peroxidase-conjugated goat anti-mouse IgG antibody or goat anti-rabbit IgG antibody (1:5000, Cell Signaling Technology (Danvers, MA, USA)) and imprinted with an enhanced chemiluminescence (ECL) detection kit (GE Healthcare, Buckinghamshire, UK).

    other:

    Article Title: Long-Fiber Carbon Nanotubes Replicate Asbestos-Induced Mesothelioma with Disruption of the Tumor Suppressor Gene Cdkn2a ( Ink4a/Arf )
    Article Snippet: Rabbit anti- p44/42 MAP kinase (phosphorylated Erk1/2) , Cell Signaling Technology , Cat# 9101; RRID: AB_331646.



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